Last edited by Goltitilar
Monday, July 27, 2020 | History

2 edition of Studies on temperature-sensitive mutants of mouse cytomegalovirus found in the catalog.

Studies on temperature-sensitive mutants of mouse cytomegalovirus

Hazem M. O. M. Akel

Studies on temperature-sensitive mutants of mouse cytomegalovirus

by Hazem M. O. M. Akel

  • 245 Want to read
  • 30 Currently reading

Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Birmingham, School of Biological Sciences, 1993.

Statementby Hazem M.O.M. Akel.
ID Numbers
Open LibraryOL21638957M

SPANDIDOS DA and Graham AF: Complementation between temperature-sensitive and deletion mutants of reovirus. Journal of Virology , 4. SPANDIDOS DA, Krystal G and Graham AF: Regulated transcription of the genomes of defective virions and temperature-sensitive mutants of reovirus. Journal of Virology , 5. The HIV-1 env protein is synthesized as a precursor (gp) that is cleaved during transport to the plasma membrane into two noncovalently associated subunits, gp, which is involved in binding to the HIV receptor, and gp41, which contains the membrane anchor and a carboxyl-terminal cytoplasmic domain of amino acids (22, 23).To determine the influence of both sequence and length of the.

Rerouting the traffic from a virus perspective. Linda Cruz 1, Nicholas J. Buchkovich 1. 1 Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA. TABLE OF CONTENTS. 1. Abstract 2. Introduction 3. . This book details the most recent research in these collective fields. In Novel Approaches to Vaccine Research, topics ranging from innovative approaches to eradicate polio to the future of.

The intrinsic pathway in C. elegans, D. melanogaster and mammalians. The functional homologues are represented in boxes with the same colour. The activation of effector caspases occurs downstream of the activation of initiator caspases (CED‐3, Dronc and caspase‐9), which occurs on the formation of the apoptosome, a protein complex contaning CED‐4, Ark/Dark and Apaf‐1, and cytochrome saltybreezeandpinetrees.com by: 2. Targeted delivery combined with controlled drug release has a pivotal role in the future of personalized medicine. This review covers the principles, advantages, and drawbacks of passive and active targeting based on various polymer and magnetic iron oxide nanoparticle carriers with drug attached by both covalent and noncovalent saltybreezeandpinetrees.com by:


Share this book
You might also like
Kenan professorships

Kenan professorships

school in transition

school in transition

Sons of vulcan

Sons of vulcan

The Tenth Annual International Workshop on Micro Electro Mechanical Systems

The Tenth Annual International Workshop on Micro Electro Mechanical Systems

Reorganization of the U.S. Fire Administration

Reorganization of the U.S. Fire Administration

Cowls

Cowls

Until the Colors Fade

Until the Colors Fade

Research to answer what Blacks ought to have

Research to answer what Blacks ought to have

Be a part of the energy market neighborhood watch

Be a part of the energy market neighborhood watch

Don Martins Droll Book

Don Martins Droll Book

All the day long

All the day long

Why Kerouac matters

Why Kerouac matters

Economic growth, Brazil, India, Japan

Economic growth, Brazil, India, Japan

Studies on temperature-sensitive mutants of mouse cytomegalovirus by Hazem M. O. M. Akel Download PDF EPUB FB2

Akel HM, Furarah AM, Sweet C () Further studies of 31 temperature-sensitive mutants of mouse cytomegalovirus: thermal stability, replication and analysis of temperature-sensitive functions by temperature shift. FEMS Microbiol Lett – PubMed CrossRef Google ScholarCited by: To study the pathogenicity of mouse cytomegalovirus (MCMV) and to identify virulence determinants, we have isolated and phenotypically characterised 25 temperature-sensitive (ts) mutants.

Urine and breast milk represent the main routes of human cytomegalovirus (HCMV) transmission but the contribution of renal and mammary epithelial cells to viral excretion remains unclear. We observed that kidney and mammary epithelial cells Studies on temperature-sensitive mutants of mouse cytomegalovirus book permissive to HCMV infection and expressed immediate.

Undoubtedly, a better understanding of the functions of the viral genome in infected and transformed cells will be achieved through studies with temperature-sensitive (ts) mutants of herpesviruses since, theoretically, any essential gene function can be affected by mutants of this saltybreezeandpinetrees.com by: @article{osti_, title = {Abundant constitutive expression of the immediate-early 94K protein from cytomegalovirus (Colburn) in a DNA-transfected mouse cell line}, author = {Jeang, K.T.

and Cho, M.S. and Hayward, G.S.}, abstractNote = {A kilodalton phosphoprotein known as IE94 is the only viral polypeptide synthesized in abundance under immediate-early conditions after infection by.

While the salivary gland has been recognized as an important effector site of the common mucosal immune system, a useful model for studying anti-viral salivary gland immune responses in vivo and for exploring the role of the salivary gland within the common mucosal system has been lacking.

Murine cytomegalovirus (MCMV) is a beta-herpesvirus that displays a strong tropism for the salivary gland Cited by: Generation of Mutant Murine Cytomegalovirus Strains from Overlapping Cosmid and Plasmid Clones Article in Journal of Virology 74(19) · November with 23 Reads How we measure 'reads'.

Murine cytomegalovirus (MCMV) is widely used as a model virus system for the study of human cytomegalovirus (HCMV) pathogenesis.

MCMV and HCMV share an essentially collinear arrangement of genes (7, 31) and similar pathogenesis in their respective hosts ().Our understanding of MCMV and HCMV biology has been greatly facilitated by the construction of recombinant viruses bearing specific. Jun 02,  · BALB/c and B6 mice were infected intranasally with either Ad-gB or FG using a dose of 10 6 PFU per mouse.

The development of antibody to MCMV gB in serum over time was assayed by IFA using A13 cells ().Hundred percent of BALB/c mice inoculated intranasally developed antibody by day 30 after immunization, while none of the mice given FG developed antibody to saltybreezeandpinetrees.com by: tsAF8 cells are temperature sensitive (ts) mutants of BHK cells that are arrested in G 1 at the non-permissive temperature.

When made quiescent by serum restriction, they can be stimulated to enter S phase by 10% serum at 34°C but not at °C. The latency and reactivation of temperature- sensitive mutants of mouse cytomegalovirus in different organs of mice.

Jordan Journal of Biological Studies, Hashemite University, Jordan. MEMBERSHIP IN PROFESSIONAL SOCIETIES AND ORGANIZATIONS 1. Pan American Society for Clinical Virology. European Society for Clinical Virology.

Jordan Journal of Biological Sciences (JJBS) The Jordan Journal of Biological Sciences (JJBS) Chapter in a book: Blaxter M. Social class and health inequalities. In: Carter C and Peel J, editors.

The Latency and Reactivation of Temperature- Sensitive Mutants of Mouse Cytomegalovirus. Generation of mutant murine cytomegalovirus strains from overlapping cosmid and plasmid clones. Generation of Mutant Murine Cytomegalovirus Strains from Overlapping Cosmid and Plasmid Clones. Our long-term interest in developing a recombinant virus generation system was to eventually construct MCMV mutants for pathogenesis studies.

Thus. One of the most fundamental questions to address when studying a viral gene is whether the gene is essential for the viral life cycle in tissue culture or tropism for a specific cell type.

At the beginning of herpesviral studies essential genes have been found by random screen of Cited by: 4. For example, BAC-directed deletion mutants are commonly used to determine the function and essentiality of viral genes. In this paper, we discuss the creation of herpesvirus BACs, functional analyses of herpesvirus mutants, and future applications for studies of saltybreezeandpinetrees.com by: Sep 25,  · Animal Virus Genetics is a collection of scientific presentations of the ICN-UCLA Symposia on Molecular and Cellular Biology, held at the University of California, Los Angeles in Book Edition: 1.

Initial attempts to identify genes essential for FV3 replication involved the isolation of a number of temperature-sensitive mutants [38,39,40]. More recent alternative ways to elucidate the function of several viral genes used transient knock down of viral gene by antisense morpholino oligonucleotides or small interfering RNA (siRNA).

However Cited by: Temperature-sensitive mtg1 mutants were obtained by low-stringency PCR amplification of the gene. The mtg1 null mutant was transformed with a library consisting of the PCR products cloned in a low-copy CEN plasmid.

Several clones were isolated that were temperature sensitive for. Targeted Lentiviral Vectors: Current Applications and Future Potential and although it seems a non-immunogenic system in several mouse strains, studies with intramuscularly envelope glycoprotein of the avian retrovirus was used to pseudotype VSV virions in order to selectively enrich for VSV temperature-sensitive mutants of Cited by: 2.

Essential genes are those genes of an organism that are thought to be critical for its survival. However, being essential is highly dependent on the circumstances in which an organism lives.

For instance, a gene required to digest starch is only essential if starch is the only source of energy. Recently, systematic attempts have been made to identify those genes that are absolutely required to. The changes in COG and GEARs induced by depleting cells of ϵ-COP were temperature sensitive (observed only after a temperature shift from 34 to °C).

This is reminiscent of the previously reported temperature-sensitive growth of ϵ-COP null yeast mutants (Duden et al., ; Kimata et al., ).

Finally, simultaneously disabling COG and.How to find your way through this book. This book explains how gene cloning, PCR and other DNA analysis techniques are carried out and describes the applications of these techniques in modern biology. The applications are covered in the second and third parts of the book.Apoptosis, derived from the Greek word for the natural process of leaves falling from trees, is a distinct form of programmed cell death (Kerr et al., ; Kerr, ).Although such programmed deaths were described many decades ago, the significance of apoptosis was largely overlooked, in particular, its relevance to disease.